Status of laboratory biosafety and biosecurity in veterinary. This biosafety manual is applicable to all laboratory, research, teaching, service, and support activities that may involveexposure to biohazard agents or materials ous or otherwisecomes under the purview of the institutional biosafety committee ibc at the university of montana. For more than 20 years, since it was first published in 1983, the laboratory biosafety manual has provided practical guidance on biosafety techniques for use in laboratories at all levels. A questionnaire was developed to obtain information from researchers across nigeria from july 2014 to july 2015. Laboratory staff, from the laboratory directors on down, need to take responsibility for the biosafety within their facilities and ensure that staff are properly trained and that their facilities meet present containment requirements. Who laboratory biosafety manual having served for global biosafety community hereinafter, who manual for more than 20 30 years, since it was first published in 1983, the laboratory biosafety manual has provided practical guidance on biosafety techniques for use in laboratories at all levels. Laboratory biosafety manual world health organisation staff. Its goal is to help prevent labacquired infections while allowing researchers to work with organisms of various levels of hazard. This biosafety manual is applicable to all laboratory, research, teaching, service. For more than 20 years, since it was first published in 1983 the laboratory biosafety manual has provided practical guidance on biosafety techniques for use in laboratories at all levels. Review of laboratory biosafety manuals and standard operating procedures sops for compliance with guidelines for bsl and absl procedures.
Illumination is adequate for all activities, avoiding reflections and glare that could affect vision. National lab biosafety and biosecurity task force to develop nlbbp administrative policy, manual of standards and guidelines task working group members of lab biosafety task force of the dept. The manual now covers risk assessment and safe use of recombinant dna technology, and provides guidelines for the comissioning and. The primary purpose of the biosafety manual is to provide an appropriate training and reference tool relative to biosafety for laboratory staff, as discussed in the ehs sop, biosafety training. Biosafety manual research and creative scholarship university.
Good microbiological technique and appropriate use of biosafety equipment by well trained staff remain the fundamental elements of laboratory biosafety. No or low individual and community risk a microorganism that is unlikely to cause. For more than 20 years, since it was first published in 1983, the laboratory biosafety manual has provided practical guidance on biosafety techniques for use in. Canadian biosafety standard cbs second edition canada.
The third edition of the who laboratory biosafety manual is a helpful reference and guide to nations that accept the challenge to develop and establish national codes of practice for securing microbiological assets, yet ensuring their availability for clinical, research and epidemiological purposes. Laboratory biosafety and biosecurity risk assessment. This manual is designed to provide general operating and emergency procedures for working safely in wesleyan university s biosafety level 2 bsl2 lab henceforth the lab. The biosafety manual provides information, guidelines, and policies that should be used in conjunction with other resources to safely work with and in the. Guidance documents manuals ebsa european biosafety. Biosafety level designations are based on a composite of the design features, construction, containment. Laboratory biosafety manual national library of australia. Access to the laboratory is restricted by the supervisor, who establishes the biosafety level, the need for specified personal protective equipment, the need for training. As needed, an adhoc committee may be formed to consider policy implications for the university of emerging technologies and processes that have a biosafety component. Biosafety level bsl and animal biosafety level absl laboratories for research and diagnostic work using developed laboratory inspection checklists. K guidance on risk assessment and cost benefit analysis.
This manual also defines the duties and responsibilities of all individuals using or operating the lab. A spill of any quantity involving an agent infectious to humans, plants, or animals occurs in a public area. Sample laboratory biosafety manual for microbiological activities. Who laboratory biosafety manual legionella testing control of.
Laboratories need leaders who can effectively utilize the laboratories resources, maximize the laboratoriescapacity to detect disease, and advocate for laboratories in. This biosafety manual has been developed by the environmental health and safety ehs department at michigan state university. A read is counted each time someone views a publication summary such as the title, abstract, and list of authors, clicks on a figure, or views or downloads the fulltext. Aug 06, 2010 laboratory biosafety manual third edition world health organization geneva 2004 slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. Biosafety manual approved july 28, 2014 page 3 of 17 tuberculosis, st. Who laboratory manual for the examination of human semen and spermcervical mucus interaction. Vacuum lines are protected with liquid disinfectant traps. Relation of risk groups to biosafety levels, practices and equipment risk group biosafety level laboratory type laboratory practices safety equipment 1 basic biosafety level 1 basic. Managing the risks in laboratories and healthcare premises, hse, u. Laboratory facilities are designated as basic biosafety level 1, basic biosafety level 2, containment biosafety level 3, and maximum containment biosafety level 4. Biosafety, in medicine and health care settings, specifically refers to proper handling of organs or tissues from biological origin, or genetic therapy products, viruses with respect to the environment, to ensure the safety of health care workers, researchers, lab staff, patients, and the general public. Major revisions to the 2014 institutional laboratory biosafety manual. The 1, 2, 3s of biosafety levels the basic layout of a bsl2 laboratory is depicted in.
Activities that are specifically addressed are those involving. Visitors have to be accompanied with someone allowed to enter and be briefed on how to behave within a laboratory. The biosafety manual is not a replacement or substitute for a general laboratory safety manual, which covers other occupational hazards in the laboratory. Who laboratory biosafety manual lbm revision update. Jun 27, 2014 biosafety is the section of environmental safety that addresses working safely in the laboratory with microorganisms and rdna organisms whose genetic material has been altered. The first edition of the world health organization who laboratory biosafety manual was published in 1983. Laboratory biosafety manual world health organization. Introduction this manual is intended to be a resource for information, guidelines, policies, and procedures that will enable and encourage those working in the laboratory environment to work safely and reduce or eliminate the potential for exposure to biological hazards. The canadian biosafety standard cbs, 2 nd edition, 2015 describes the minimum requirements for containment zones in a regulated facility. This is the third, revised edition of the booklet biosafety in the laboratory. The laboratory biosafety manual should be a living, working document that is an important resource for personnel engaged in the activities using biological and or rdna materials.
A manual of written standard operating procedures sops for the laboratory, in combination with this manual and the cdcnih publications biosafety in microbiological and biomedical laboratories, 5th edition and guidelines for research involving recombinant or synthetic nucleic acid molecules, provide general requirements for working with biological agents. Who laboratory manual for the examination and processing of human semen 5th ed. Its primary focus should be to provide pertinent information to help execute the operations of the lab in a safe and professional manner. Only a few alterations have been made, especially in the text concerning decontamination and inactivation. Laboratory biosafety manual were published in 1993 and 2004 respectively 2,3. Laboratory biosafety manual standard specifications for language laboratory microform language laboratory facilities, technical guide for the selection, purchase, use, and maintenance, study. Good microbiological technique and appropriate use of biosafety equipment by welltrained staff remain the fundamental elements of laboratory biosafety.
Receive training in standard microbiological techniques. The ibc advocates the use of biosafety precautions that effectively reduce or. Who laboratory manual for the examination and processing. The manual is part of msus biosafety program, which was established to accomplish the following goals. Manual, the us biosafety in microbiological and biomedical laboratories, and the international consensus document cen workshop agreement cwa 15793. The biosafety manual also intends to aid in the protection of laboratory personnel and the surrounding environment from possible exposure to biohazardous material. The development of this fourth edition of the laboratory biosafety manual has. It is the responsibility of the laboratory director the person who has immediate responsibility for the laboratory to ensure the development and adoption of a biosafety management plan and a safety or operations manual. Information regarding demographics, knowledge of laboratory biosafety, availability and proper use of personal protective equipment ppe, any priority pathogens researched, attitude on and use of standard laboratory practices, and biosafety awareness was obtained using a.
In this booklet you will find all the information that was already present in the earlier editions. Biological laboratory safety training risk group classification nih guidelines for research involving recombinant dna molecules, 2011 who laboratory biosafety manual, 3rd edition risk group 1 agents that are not associated with disease in healthy adult humans. Dec 07, 2010 if you would like to receive updates as the work on the biosafety manual progresses. A laboratory acquired infection is known or suspected, or b.
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